Project
Characterizing replicating bacteriophages in the gut using amino acid tagging and "click" chemistry.My project aims to illuminate the dynamics of the gut virome, particularly focusing on actively replicating phages and their interplay with bacterial communities. We propose a novel methodology, employing BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT) with methionine analogs, to identify and characterize replicating phages in the gut. Through this, we anticipate uncovering temporal and inter-individual variations in the replicating phage subset.
