ProjectDysbiosis Induction in Aedes aegypti to reduce Virus Transmission Efficacy: from natural microbiome diversity to lab experiments
This project gathers six experts in the fields of entomology, microbial ecology and biogeochemistry from Guyana and Canada to conduct a high-‐impact, holistic investigation of Aedes aegypti holobiont thriving in anthropogenic environments and provoking increasing arboviruses epidemics. Two experimental approaches, combining field and laboratory populations of Ae. Aegypti, will be used to demonstrate the deterministic effect of microbiome structure of larvae and adults on insecticide resistance and life history traits. Firstly, a field survey will be performed to characterize the bacteriome and mycobiome of natural Ae. aegypti populations. This result will be analyzed to infer the impact of biotic and abiotic features of water breeding sites on the structure of Ae. Aegypti microbiota and identify bacterial and fungal species whose distribution is related to the phenotypes. A genomic barcode to predict the selection pressure of breeding sites on Ae. Aegypti populations with one or another phenotype will be developed. Using synthetic microbial consortia, the second objective is aimed at evaluating the impact of microbiota dysbiosis on the mosquito population’s resistance and larval history traits.