High throughput sequencing (NGS)

Massively parallel sequencing methods (also called next-generation sequencing NGS) are becoming the standard for genetic diversity and molecular evolution studies, but designing these experiments can be intimidating.

This training session consist in a 30-minutes presentation, where the characteristics of the most common sequencing platforms are described, and most importantly, and where the main laboratories steps and expenses are summarized for five case studies. A discussion period follows. The target audience is QCBS researchers and students who might eventually want to use these technologies for their future research projects, but are not familiar with the molecular steps and costs involved. Please contact Annie Archambault to indicate your interest for this presentation.

An optional practice session is possible where participants would work with a set of data available on GenBank SRA, and analyze the short reads using freely available software. This second part of the training may take place in 2013.

List of the cases studies discussed in the presentation